hexokinase type iv from bakers yeast
Hexokinase from Saccharomyces cerevisiae
CAS: 9001-51-8
Molecular Formula: N/A
hexokinase type iv from bakers yeast - Names and Identifiers
hexokinase type iv from bakers yeast - Physico-chemical Properties
| Molecular Formula | N/A |
| Molar Mass | 55kDa (SDS-PAGE) |
| Water Solubility | Soluble in water. |
| Appearance | White to white-like powder |
| Storage Condition | -20°C |
| Sensitive | Hygroscopic |
| MDL | MFCD00131321 |
| Physical and Chemical Properties | Hexokinase is a fine needle-like crystal. Commercially available are freeze-dried powder, 70% saturated ammonium sulfate crystallization suspension and 50% glycerol solution. 12 months without reducing the activity of the phenomenon, the county floating liquid should prevent freezing. |
| Use | It is an enzyme that catalyzes the phosphorylation of hexose from ATP at the 6 position. The affinity for α-D glucose is very high (Km = 1 × 10-5M), and other hexoses can also be phosphorylated. The balance of this reaction is shifted to the right. △ g '= 4.0 kcal. The enzyme can be activated by Mg2 and K, but can be inhibited by HS reagents. |
hexokinase type iv from bakers yeast - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| FLUKA BRAND F CODES | 10-23 |
| TSCA | Yes |
| HS Code | 35079090 |
hexokinase type iv from bakers yeast - Trait
White powder.
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Vitality definition
One unit will convert one micromole of D-Hexose to D-Hexose-6-phosphate per minute at pH 8.0 at 30℃.
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Source
Saccharomyces cerevisiae extract.
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Isoelectric point
5.5。
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Optimum temperature
50℃~55℃。
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Optimum pH
9.0。
Last Update：2023-08-16 21:32:38
hexokinase type iv from bakers yeast - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| introduction | hexokinase (Hexokinase) is one of the key enzymes in the respiratory metabolism of organisms, which can catalyze hexose phosphorylation. Hexokinase exists in all cells and is an enzyme needed by cells to produce energy. There are 5 subtypes of , of which I and II are distributed in the outer membrane of mitochondria and bind to voltage-dependent cation channel proteins; this part allows hexokinase to directly obtain ATP produced by mitochondria. ATP is one of the substrates of hexokinase. |
| physiological function | hexokinase plays a pattern recognition receptor function in the innate immune system, similar to the gatekeeper role of the immune system, and has important functions. This mechanism exists not only in animal cells, but also in human cells. Therefore, hexokinase is often used as an indicator to monitor human health. At present, hexokinase has been used in blood glucose detection kits. |
| use | commonly used diagnostic enzymes. Biological research. The coupling reaction of glucose level, ATP level and creatine kinase in biological fluid was verified. used in biochemical research, clinically commonly used as diagnostic enzymes, for glucose analysis, preparation of urine sugar and blood sugar test paper, pharmaceutical industry used as a stabilizer of vitamin C and B12 preparations can also be used for protein sugar removal, canned wine storage. |
| production method | yeast is used as raw material, sodium phosphate is extracted and graded with ammonium sulfate, and then the product is obtained by dialysis, DEAE-Sephadex column chromatography, DEAE-cellulose column chromatography, P-I and P-II hydroxyapatite column chromatography, and ammonium sulfate recrystallization. |
Last Update：2024-04-09 20:52:54
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